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VIENAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF AGRONOMY UNDERGRADUATE THESIS TOPIC: STUDY ON THE TECHNICAL MEASURES FOR THE CULTIVATION OF NGOC LINH GINSENG (PANAX VIETNAMENSIS) IN VITRO SEEDLING IN NURSERY Student : Vu Thi Huyen Trang Student code : 611646 Class: K61-KHCTT Supervisor : Dr. Thieu Thi Phong Thu Assoc.Prof.Dr. Le Hung Linh Department : Crop Science HA NOI -2021
ACKNOWLEDGE There is an undeniable fact that, beside the own effort, there are no successes that are not associated with support or assistance whether more or less, directly or indirectly by others. This thesis would not have been possible if I did not have support of many individuals and organizations. Therefore, I would like to extend my sincere gratitude to all of them. First of all, I would like to express my thankful to the lecturer in Faculty of Agronomy of Vietnam National University of Agriculture with their knowledge and dedication to convey valuable knowledge to me during the time of studying at university. Secondly, I am using this opportunity to express my deep and sincere gratitude to Dr. Thieu Thi Phong Thu and Associate prof. Dr. Le Hung Linh who in site of being extraordinarily busy with her duties, took time out to hear, guide and keep me on the correct path. It was a great privilege and honor to work and study under their guidance I am also grateful to Department of Molecular Biology - Institute of Agricultural Genetics, Experimental station in Van Giang - Hung Yen and technical support in the implementation. I would not forget to express my special thanks to Dr. Khuat Thi Mai Luong and all personnel of Institute. Without Dr. Khuat Thi Mai Luong and others superior knowledge and experience, this undergraduate thesis would not like in quality of outcomes, and thus their guidance and support have been essential. However, due to limiting my professional knowledge and lacking in practical experience, the content of the topic can not avoid the deficiencies. I look forward to receiving recomments from lecturers and all staffs at the university that make this report complete. Thank you for your consideration. Hanoi, March …. 2021 i
TABLE OF CONTENTS ACKNOWLEDGE ....................................................................................................... i TABLE OF CONTENTS............................................................................................. ii LIST OF TABLE........................................................................................................ iv LIST OF GRAPH .........................................................................................................v LIST OF FIGURE ...................................................................................................... vi LIST OF FIGURE ...................................................................................................... vi ABSTRACT .............................................................................................................. vii PART 1: INTRODUCTION.......................................................................................1 1.1. Background...........................................................................................................1 1.2. Objectives and requirements .................................................................................2 1.2.1. Objectives ..........................................................................................................2 1.2.2. Requirements .....................................................................................................2 PART 2: LITERATURE RVIEW .............................................................................3 2.1. Introduce about Ginseng .......................................................................................3 2.2. General introduction to ginseng species of the Panax genus in Vietnam ...............7 2.3. Introduce about Ngoc Linh ginseng ....................................................................11 2.3.1. The original of Ngoc Linh ginseng...................................................................11 2.3.2. Botanical characteristics, growth and development of Ngoc Linh ginseng, external requirements of Ngoc Linh ginseng. .............................................................13 2.3.3. Natural conditions in the distribution area of Ngoc Linh ginseng Climate condition ................................................................................................................16 2.3.4. The value of Ngoc Linh ginseng ......................................................................17 2.4. The state of Ngoc Linh ginseng research and development .................................18 2.4.1. Researches on propagation from seeds .............................................................19 2.4.2. Researches on in vitro clonal propagation .......................................................19 2.5. Researches optimize the growth and development process of Ngoc Linh ginseng. ....20 2.5.1. The condition of planting and caring for Ngoc Linh ginseng ............................20 2.5.2. Research on growing medium Ngoc Linh ginseng ...........................................21 ii
PART 3. CONTENT AND METHOD OF RESEARCH........................................25 3.1. Research materials ..............................................................................................25 3.2. Place and time of the research .............................................................................27 3.3. Research content.................................................................................................27 3.4. Experimental parameters.....................................................................................29 3.5. Data processing methods.....................................................................................29 PART 4. RESULTS AND DISCUSSION ................................................................30 4.1. Research on standardization of in vitro seedlings Ngoc Linh ginseng .................30 4.2. Effect of the substrate on the viability and growth of Ngoc Linh ginseng in vitro seedlings....................................................................................................................34 4.2.1. Experiment in laboratory conditions at Van Giang Experimental Station .........34 Figure 4. 5: Ngoc Linh ginseng in vitro seedling used in the experiment.....................35 4.2.2. Experiment in net house conditions at the Experimental station in Sa Pa..........37 4.2.3. Experiment in nursery conditions at Sa Pa experimental station .......................39 4.3. Effect of plant age on in vitro seedling adaptability in vitro in nursery ................42 4.4. Effect of spraying time of AT Chibozin on the growth of in vitro seedlings in the nursery.......................................................................................................................46 4.5. Assessment of pests and diseases of Ngoc Linh ginseng in Sa Pa........................49 PART 5. CONCLUSIONS AND SUGGESTIONS .................................................53 5.1. Conclutions.........................................................................................................53 5.2. Suggestion ..........................................................................................................53 REFFERENCE.........................................................................................................54 ACTIVE IMAGE......................................................................................................56 iii
LIST OF TABLE Table 2.1: Species of ginseng were registered...............................................................3 Table 2.2: Panax species in the world ...........................................................................6 Table 4.2: Effects of substrate on in vitro seedling adaptability in the net house conditions...................................................................................................................38 Table 4.3: Table 4. 3 Effects of substrate on in vitro seedling adaptability in nursery conditions ...................................................................................................................40 Table 4.4: Effect of plant age on in vitro seedling adaptability in nursery ...................44 Table 4.5: Effect of spraying time of AT Chibozin on the growth of in vitro seedlings in the nursery..............................................................................................................48 iv
LIST OF GRAPH Graph 4.1: Describe the survival rate of Ngoc Linh ginseng in vitro seedling planting in three location ..........................................................................................................42 Graph 4.2: Effect of plant age on in vitro seedling adaptability in nursery ..................45 v
LIST OF FIGURE Figure 2.1: The most commonly of ginseng .................................................................4 Figure 2.2: Distribution of Ginseng species in the world...............................................5 Figure 2.3: Ngoc Linh ginseng....................................................................................15 Figure 3.1: Ngoc Linh ginseng in vitro seedling..........................................................25 Figure 3.2: Perlite rock and Vermiculite of Namix company......................................26 Figure 4.1: Ngoc Linh ginseng tubers have no morphogenetic differentiation.............30 Figure 4.2: The ginseng tubers have not split clearly between buds and node...................32 Figure 4.3: Ngoc Linh ginseng tuber is morphogenetic differentiation........................32 Figure 4.4: Ngoc Linh ginseng tuber have morphogenetic differentiation clearly........33 Figure 4.1: Ngoc Linh ginseng in vitro seedling used in the experiment .....................35 Figure 4.6: Ngoc Linh ginseng in vitro seedlings in the net house...............................37 Figure 4.7: Ngoc Linh ginseng in vitro seedlings in Sapa...........................................39 Figure 4.8: Ngoc Linh ginseng in vitro seedlings growth in Sapa................................45 Figure 4.9: Experiment spraying AT Chibozin after 20 days planting .........................47 Figure 4.10: Photos of bare slugs harming Ngoc Linh ginseng seedling in Sa Pa........51 Figure 4.11: Image of diseases of Ngoc Linh ginseng seedlings in Sa Pa ....................52 vi
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