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VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF BIOTECHNOLOGY UNERGRADUATE THESIS ANALYSIS OF GENETIC DIVERSITY OF LOCAL PIGMENTED RICE IN VIETNAM Student : NGUYEN VU KIEU OANH Supervivor : LE DUC THAO, Ph.D MSc. NGUYEN QUOC CHUNG ID : 614076 Class : K61CNSHE Hanoi - 2021
ACKNOWLEDGEMENTS Firstly, I would like to express my appreciation to the directory of the Vietnam National University of Agriculture, the board of deans, and lectures in the faculty of biotechnology that created the best condition for me to perform and complete my thesis. Secondly, I’m deeply indebted to my supervisors, Dr. Le Duc Thao and MSc. Nguyen Quoc Trung, who guided me to conduct experiments and advised me on professional knowledge. Their support gave me knowledge and patience to accomplish the thesis. I would also like to thank guides from Crop Research and Development Institute who shared useful experiences in building field layout and rice cultivation for me. I gratefully acknowledge my colleagues in the laboratory for their wonderful assistance. Thank to them, I can complete my research with high excitement. Last but not least, I would like to thank my parents for their support in the study as well as in life. I have never finished my dissertation without their love and their support. I sincerely thank all of you! Hanoi ……………… Student Nguyen Vu Kieu Oanh i
COMMITMENT I guarantee that this thesis is my original works and all the data and results in this thesis are truthful and have never been used in any report yet. I also assure that the information cited in the thesis is indicated the origin and all the help is thankful. Hanoi ……………… Student Nguyen Vu Kieu Oanh ii
LIST OF CONTENTS Acknowledgements................................................................................................i COMMITMENT...................................................................................................ii LIST OF contents.................................................................................................iii LIST OF TABLES................................................................................................v LIST OF FIGURES .............................................................................................vi SUMMARY........................................................................................................vii CHAPTER 1. INTRODUCTION.........................................................................1 1.1. Introduction....................................................................................................1 1.1.2.Objectives ....................................................................................................2 1.1.3. Requirements ..............................................................................................2 CHAPTER 2. LITERATURE REVIEW..............................................................3 2.1. Genetic resources of rice................................................................................3 2.1.1. Genetic resources........................................................................................3 2.1.2. Global genetic resources of rice .................................................................3 2.1.3. Genetic resources of rice in Vietnam..........................................................6 2.1.4. Pigmented rice genetic resources................................................................6 2.2. Pigmented rice ...............................................................................................7 2.2.1. Properties of pigmented rice.......................................................................7 2.2.2. Anthocyanin................................................................................................9 2.2.3. The studies about genetic diversity of rice...............................................14 Chapter 3. Materials and methods. .....................................................................16 3.1. Materials ......................................................................................................16 3.2. METHODS..................................................................................................19 3.2.1. Field layout ...............................................................................................19 3.2.2. DNA extraction with potasium acetate (CH3COOK)...............................20 3.2.3. PCR reaction:............................................................................................20 3.2.4. Electrophoresis:.........................................................................................21 3.2.5. Data processing.........................................................................................21 iii
3.2.6. Anthocyanin content measurement...........................................................22 Chapter 4. Results and discussions.....................................................................24 4.1. The result of the DNA extraction of 30 accessions.....................................24 4.3. Analysis the genetic relationships between the studied pigmented rice samples. ....................................................................................................27 4.3. Total anthocyanin content............................................................................28 CHAPTER 5. CONCLUSIONS AND SUGGESTIONS...................................32 5.1. Conclusions..................................................................................................32 5.2. Suggestions..................................................................................................32 REFERENTS......................................................................................................34 APPENDIX.........................................................................................................42 iv
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